Meet Mitopure^®The first clinically tested Urolithin A supplement

Muscle health and cellular energy naturally decline with age, often beginning in our 30s. Urolithin A is a naturally occurring postbiotic that supports mitophagy, the process that renews mitochondria and helps cells produce energy more efficiently. Across 25+ human clinical studies, including multiple randomized, double-blind, placebo-controlled trials, Urolithin A has been shown to support key pillars of healthy aging: muscle performance and endurance, reduced fatigue and improved recovery, immune resilience, and skin health.

How this translates into real-life benefits:

- Muscle and performance: Supports muscle endurance and mitochondrial health in mid-life and older adults, and has been studied in active populations.

- Fatigue and recovery: Shown to improve ratings of perceived exertion and markers linked to exercise recovery.

- Immune support: Clinical trials report improvements in immune cell function and inflammatory markers associated with immune aging.

- Skin: Clinical studies, including topical applications of Mitopure, show improved mitochondrial function in skin cells and optimized collagen assembly, resulting in visible improvements in wrinkles, hydration, and skin resilience to UV stress.

In clinical studies, Mitopure® was shown to induce a selective recycling of damaged mitochondria, a process known as mitophagy. This is a natural process in our cells that cleans up defective and unhealthy mitochondria. Mitophagy becomes less efficient as we age. A decline in mitophagy and mitochondrial function are key drivers of the aging process. (Ryu et al, 2016).

Urolithin A is generated by the gut microflora as a natural food metabolite of ellagitannins, a class of compounds found in the pomegranate and other fruits and nuts. Sources of ellagitannins include: pomegranates, nuts, some berries (raspberries, strawberries, blackberries, cloudberries), tea, muscadine grapes, tropical fruits, and oak-aged wines. However, consuming these foods does not guarantee the transformation of ellagitannins into Urolithin A.

Conversion rates vary from person to person. Upon consumption of pomegranate juice, for example, compounds known as ellagitannins are broken down in the stomach and transformed by intestinal bacteria into Urolithin A. This biotransformation has been shown to vary widely across individuals, with some showing high or low conversion rates, while others are unable to perform the conversion at all. (García-Villalba R et al, 2017).

We have performed extensive preclinical studies demonstrating the safety of Mitopure® for human consumption (Heilman et al, 2017). In addition, since 2016, we have performed over 7 nutritional clinical studies, and over 12 skincare clinical studies with our Mitopure® demonstrating its safety in humans (Singh et al, 2017; Liu et al, 2022; Singh et al 2022). Mitopure® has also been favorably reviewed by the U.S. Food and Drug Administration (FDA) following a GRAS (generally recognized as safe) filing.

No Mitopure®-related adverse events have been reported when taking Mitopure® at the recommended dosage for healthy adults over 18 years of age. If you experience any side effects from taking Mitopure®, please email us at care@timeline.com or call +1 888-631-3359. Our team is available to help Monday through Friday, 9am to 5pm EST.

Always consult with a healthcare professional before starting supplements, especially if you have any pre-existing health conditions or are taking medications. Mitopure® is not recommended for use during pregnancy, breastfeeding, or in children without medical advice.

The science was developed by the Timeline Longevity research team at Amazentis SA, in collaboration with the Swiss Federal Institute of Technology (EPFL) and leading longevity MDs and scientists. Timeline Longevity’s co-founders are MDs and PhDs and the company employs more than 6 PhDs, an MD and 3 Registered Dietitians.

Mitopure products have been on the market since July 2020. Mitopure is the #1 Doctor Recommended Urolithin A brand and products are available to purchase directly at timeline.com.

Yes, Mitopure® is a proprietary brand name for Urolithin A, but it differs from generic sources because it is synthesized at >99% purity, guaranteeing a precise, effective dose most cannot get from diet alone. Mitopure® is clinically validated, patented, and manufactured to a higher standard than generic Urolithin A. Mitopure® is also the number 1 doctor recommended Urolithin A brand.

Developed by Timeline Longevity after over 18 years of research, Mitopure® is the only clinically proven Urolithin A supplement available on the market. Mitopure® (Urolithin A) has been evaluated in multiple human trials across a diverse population, demonstrating meaningful health outcomes and verified purity, stability, and bioavailability. It has been successfully notified to the FDA as a GRAS (Generally Recognized as Safe) ingredient and is NSF Contents Certified, unlike many generic alternatives, which may lack rigorous safety testing.

User experiences suggest increased and sustained energy, reduced muscle fatigue during exercise, and faster recovery after physical activity, though these outcomes have not been confirmed in clinical studies. Reported effects tend to be cumulative and are typically noticed after 2–4 months.

While everyone may not physically feel the benefits, our clinical trials have shown that Mitopure® targets a key driver of aging, triggering the mitochondrial recycling process, called mitophagy, with sustained mitochondrial health benefits.

Mitopure® works at the foundation, improving mitochondrial quality itself rather than stimulating energy production temporarily. Unlike CoQ10, PQQ, Nicotinamide Riboside, NAD+, NMN, Mitopure® primarily focuses on recycling dysfunctional mitochondria and cleaning up the old, faulty ones (mitophagy) first, preventing cellular waste accumulation. Mitopure® also helps to generate new, healthier mitochondria (biogenesis).

Both Mitopure® and NAD+ support mitochondrial health, but they work through distinct pathways.

Mitopure® works at a foundational level by removing damaged mitochondria and supporting the renewal of healthier, more efficient ones. This essential cleanup process, called mitophagy, naturally declines with age, allowing damaged mitochondria to build up and interfere with normal cellular function. By helping restore this cleanup process, Mitopure® supports a healthier population of mitochondria inside cells, enabling cells to produce energy more effectively.

NAD+, and its precursor molecules NMN and NR, support cellular energy and repair pathways, helping healthy mitochondria generate energy more efficiently. NAD+ will not facilitate the removal of damaged mitochondria.

Mitopure® supports long-term mitochondrial health by activating mitophagy, the body's natural process for removing and replacing damaged mitochondria. By helping to restore this cleanup process, Mitopure® supports a healthier pool of mitochondria inside cells, enabling them to produce energy. Clinical studies have demonstrated improvements in cellular energy and muscle strength, even without a change in exercise routine.

Creatine supports short-term energy production, helping to fuel muscles for short, explosive bursts of power. Taken alone, without strength training, creatine has minimal impact on muscle quality.

Timeline’s Mitopure® supplement is available as Softgels, Powder and Gummies.

How to Take Mitopure®: For the best results, take Mitopure® daily, ideally at the same time every day. Mitopure® can be taken at any time during the day, and you can take Mitopure® with or without food.

Recommended Dose: Mitopure® is most effective at doses of 500mg or 1000mg per day.

Yes, you can switch between forms. Remember, though, that two softgels and two gummies provide 500mg of Urolithin A while one stick pack of powder provides 500mg of Urolithin A.

For optimal benefits, based on our clinical trials, we recommend you take Mitopure® for a minimum of 4 months to support mitochondrial function and muscular health. Many of our customers choose to stay on Mitopure® indefinitely to support their long-term mitochondrial health.

Yes, Mitopure® is designed for ongoing daily use to support long-term cellular health. Mitophagy (triggered by Mitopure® (Urolithin A)) is an ongoing cellular process; stopping supplementation will likely cause Urolithin A levels to drop, returning mitochondrial recycling to its baseline (often sluggish) rate.

Doses up to 1,000mg daily have been clinically studied and found safe and effective. There are no clinical studies showing benefits beyond 1,000mg per day. The recommended daily dosage is 500mg (maintenance) to 1,000mg (clinical strength).

Yes, Mitopure® can be taken with or without food. Mitopure® can also be taken with other supplements. Consult a healthcare practitioner before using this product if you are planning on becoming pregnant, pregnant, nursing, anticipate surgery, taking medication on a regular basis, or are otherwise under medical supervision.

Yes. Mitopure® is designed for safe, daily, long-term use.

Yes, Mitopure Softgels are compatible with fasting, and all of our formulations are appropriate for keto, vegan, and other diets. For complete information, please consult the product’s Supplement Facts panel.

Likely not. Urolithin A is not directly found in foods, instead we need to rely on our gut microbiome to make it for us. Only 30–40% of the population possesses the specific gut microbiome required to convert dietary precursors (ellagitannin-rich foods, such as pomegranates and nuts) into Urolithin A, and even then, conversion is inconsistent. Additionally, most people are not consuming enough of the dietary precursors to make meaningful amounts of Urolithin A. We demonstrated in a clinical study that you would need to drink 6 glasses of pomegranate juice per day to get the equivalent of a 500mg dose.

Our Mitopure® softgels have a neutral flavor, our Mitopure® gummies have a delicious (sugar-free) strawberry flavor, and our Mitopure® powder has a tasty berry-flavor.

Store the bottle in a cool, dry place away from direct sunlight. Always keep the lid tightly closed when not in use.

Yes, our Mitopure Softgels and Powder are NSF Certified for Sport®, meaning they are rigorously tested for banned substances, and are safe for use by professional athletes. Our Mitopure Gummies are NSF content certified.

You should consult your healthcare provider. Clinical studies on Mitopure® have not specifically included pregnant or breastfeeding women.

Mitopure® is well-tolerated. In clinical trials, no serious adverse effects were reported. Mild gastrointestinal discomfort is rare but possible when starting any new supplement.

If you experience any side effects from taking Mitopure®, please email us at care@timeline.com or call +1 888-631-3359. Our team is available to help Monday through Friday, 9am to 5pm EST.

While no specific negative interactions are known, it is always recommended to consult your doctor before adding a new bioactive supplement to a prescription regimen.

Mitopure® is free from common allergens, including gluten and dairy.

Research includes over 18 years of preclinical work and multiple human clinical trials demonstrating improvements in muscle strength, VO2 max, and mitochondrial biomarkers.

Yes, Mitopure® has undergone multiple randomized, double-blind, placebo-controlled human clinical trials, which are the gold standard for scientific validation. These studies have been published in high-impact, peer-reviewed medical journals.

Yes, Timeline Longevity actively conducts new trials, in partnership with top research institutions around the world, to explore benefits in other areas such as immune health, skin longevity, and cognitive function. Keep up to date with the ongoing research on Mitopure.

The active ingredient is pure Urolithin A. Mitopure® supplements contain Urolithin A plus minimal supporting ingredients, depending on format. For complete information, please consult the product’s Supplement Facts panel.

Timeline Longevity is deeply committed to high standards for quality, safety, and transparency in every product we make. Mitopure® is manufactured under FSSC 22000 & strict cGMP (current Good Manufacturing Practices) standards and undergoes third-party testing for identity, authenticity, purity and safety.

Dietary supplements are not "approved" by the FDA like drugs, but Mitopure® has achieved FDA GRAS status, meaning the FDA has reviewed its safety dossier and declared it as safe for human consumption at the adequate daily dose.

FDA GRAS: Confirmed safety.

FSSC 22000: Food safety certified

NSF Certified for Sport®: Confirmed free of banned substances on end products containing Mitopure

Clean Label Project: Tested for contaminants on end products containing Mitopure